2 replies to this topic

[BiologyGeek95]

Hey,
I actually have a problem.
I transformed the DH5 alpha.
It was supposed to become luminescent and not grow on amp plates.
My strain did?

Any suggestions to this problem?

Thanks

[seanspotatobusiness]

I don't understand. What did you transform them with? Did you have an insert disrupting an ampicillin resistance gene? Otherwise, why would you try to grow your transformed bacteria on a medium which (you thought) would kill them?

Edited by seanspotatobusiness, 24 January 2011 - 01:58 PM.

[pito]

DH5 alpha doenst have an amp resistance gene...
How can you make it resistance then without adding this gene by introducing a plasmid or transformation of the DNA with this gene.