2 replies to this topic

[conmalone]

Hi there,

I'm about to start qPCR on HeLas and human corneal epithelial cells, looking for TLR expression. Before ordering in the TaqMan primers, I need to know whether to use FAM or VIC labelling - has anyone any ideas or suggestions?

Thanks!

Edited by conmalone, 23 January 2011 - 08:16 AM.

[ivanbio]

Both FAM and VIC will work just fine. I would recommend starting with FAM since you will always get the highest signal with FAM.

Good luck

[conmalone]

That's great - thanks a million!