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FAM and VIC labelling


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#1 conmalone

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Posted 23 January 2011 - 08:16 AM

Hi there,

I'm about to start qPCR on HeLas and human corneal epithelial cells, looking for TLR expression. Before ordering in the TaqMan primers, I need to know whether to use FAM or VIC labelling - has anyone any ideas or suggestions?

Thanks!

Edited by conmalone, 23 January 2011 - 08:16 AM.


#2 ivanbio

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Posted 23 January 2011 - 08:26 AM

Both FAM and VIC will work just fine. I would recommend starting with FAM since you will always get the highest signal with FAM.

Good luck

Ivan
Carlsbad, CA

#3 conmalone

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Posted 24 January 2011 - 01:04 AM

That's great - thanks a million!




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