Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Measuring hydrogen peroxide using fluorometer

  • Please log in to reply
1 reply to this topic

#1 anonymous



  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts

Posted 27 August 2001 - 09:00 PM

Hello. I'm trying to measure ROS, esp. hydrogen peroxide using fluorometer which called TD-700. To visualize ROS, I use DCF(dichloroflurescin diacetate). However, I have some problems: which buffer should I use when I lyse the cell, how I should set the standard in order to quantify the amount of ROS, and etc. Please help me if there is anyone who had experience in measuring the ROS using this kind of equipment...

#2 kishorebio



  • Members
  • Pip
  • 4 posts

Posted 02 September 2003 - 07:23 AM

U can use 0.1M of Phosphate buff of 7.4 pH.
First off all try to std this method in ur lab
For standardization u should take different con of H2O2 along with DCFDA
U can quantify the DCF
Ext:503 Slit:5
Em:529 Slit:10

Have more doubts feel free to contact me
All the best

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.