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Having trouble with stripping PVDF membrane during WesterDot blot


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#1 Niki Argentou

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Posted 22 January 2011 - 05:26 AM

Hello, I am wondering if anyone has faced problems with stripping PVDF membranes after using a UV transilluminator imaging system. I use a commercially available stripping solution for 20min but i couldn't remove the stain at all. Do you have any idea about what the problem is?
Thank you in advance

#2 Liopleurodon

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Posted 01 March 2011 - 07:30 AM

Hi, please give us some more details.

Exactly what chromogenic system are you using?
Has the membrane been dry at the meantime?
How long do you leave it under the illuminator?




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