interpretation of results of methylight run
Posted 21 January 2011 - 11:05 AM
I'm gonna detect methylation pattern of a gene. I've chose the methylight assay. It is a real time PCR assay which is preceded by bisulfit modification of the DNA.
The trouble is that i don't know how i'm gonna interpret the results. should i use a housekeeping gene to calculate the ∆ct. how could i get the ratio ?
Posted 06 August 2013 - 02:55 PM
hi, have you the awnser??, because I'm doing the same, if know something, please tell me.