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interpretation of results of methylight run


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#1 marium

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Posted 21 January 2011 - 11:05 AM

Hi everybody,
I'm gonna detect methylation pattern of a gene. I've chose the methylight assay. It is a real time PCR assay which is preceded by bisulfit modification of the DNA.
The trouble is that i don't know how i'm gonna interpret the results. should i use a housekeeping gene to calculate the ∆ct. how could i get the ratio ?

#2 Nyquist

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Posted 06 August 2013 - 02:55 PM

hi, have you the awnser??, because I'm doing the same, if know something, please tell me.

 

Best Regards

 

René






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