Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How to obtain kill curves for E.coli.


  • Please log in to reply
No replies to this topic

#1 DGM

DGM

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 21 January 2011 - 06:35 AM

Hey guys,

I'm currently studying Adv. Higher Biology and am in the process of planning my investigation.

I am planning on using the title "Discuss the effect of temperature on transformation of E.coli K12."

My basic method is going to include making the bacteria competent and then somehow obtaining cephalexin resistant plasmids and transforming the E.coli.

However, we get to my main question: How do I obtain a killing curve for cephalexin. If somebody to tell me a general method then that would eb great. I have a basic idea: grow the bacteria, plate them in conditions with differing antibiotic concentrations and then noting the number that survive. Couls any of you guys give a more detailed plan?

Also, if any of you know where to get cephalexin resistant plasmids or any other antibiotic resistant plasmids then if you could let me know then that would be awesome!

Thanks guys

Danny




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.