It is well-known that taq DNA polymerase makes errors during PCR, the reason for which proof reading enzymes like Pfu DNA polymerase are preferred. How can the bacteria compensate the errors made by Taq polemerase as compared those harboring proofreading enzymes? Is it that Thermus aquaticus has other proofreading enzymes and that pyrococus species having Pfu doesn't need such enzymes?
Taq DNA polymerase
Started by ram, Jan 21 2011 03:41 AM
2 replies to this topic
#1
Posted 21 January 2011 - 03:41 AM
This might appear a foolish question
It is well-known that taq DNA polymerase makes errors during PCR, the reason for which proof reading enzymes like Pfu DNA polymerase are preferred. How can the bacteria compensate the errors made by Taq polemerase as compared those harboring proofreading enzymes? Is it that Thermus aquaticus has other proofreading enzymes and that pyrococus species having Pfu doesn't need such enzymes?
It is well-known that taq DNA polymerase makes errors during PCR, the reason for which proof reading enzymes like Pfu DNA polymerase are preferred. How can the bacteria compensate the errors made by Taq polemerase as compared those harboring proofreading enzymes? Is it that Thermus aquaticus has other proofreading enzymes and that pyrococus species having Pfu doesn't need such enzymes?
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.
#2
Posted 22 January 2011 - 12:54 AM
In harsh environments a high evolutionary rate is an advantage as the bacteria can evolve to suit the changing conditions faster. For this reason Taq is actually an advantage for the species.
#3
Posted 22 January 2011 - 01:42 AM
bob1, on 22 January 2011 - 12:54 AM, said:
In harsh environments a high evolutionary rate is an advantage as the bacteria can evolve to suit the changing conditions faster. For this reason Taq is actually an advantage for the species.
Thanks for giving it a thought bob
But when we for instance compare between Thermus aquaticus and Pyrococus furiosus which both are extremophiles, how can such difference be explained?
Edited by ram, 22 January 2011 - 01:43 AM.
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.














