Loss of protein upon concentration
Posted 20 January 2011 - 09:53 PM
Posted 21 January 2011 - 01:40 PM
you may want to try a membrane with a lower mw cutoff (i would use 10 kDa or less). if your protein is not globular then it may be passing through the pores of the membrane.
genius does what it must
i do what i get paid to do
Posted 22 January 2011 - 01:25 AM
Posted 22 January 2011 - 08:21 PM
Posted 09 February 2011 - 02:05 AM
No idea how that can happen! The protein concentrated fine before, but I lost it after concentrating for a second time (using the same concentrator). Note, no precipitates formed and I'm using 10% glycerol in my buffer as well. Even stranger is the fact that what went into the gel fil definitely didn't come out into my collection tubes...
Any comments would be appreciated!
Edited by SebS, 09 February 2011 - 02:07 AM.