Posted 20 January 2011 - 09:04 PM
Posted 21 January 2011 - 12:21 AM
Posted 21 January 2011 - 11:14 AM
can you gimee the protocol for preparation of lysine coated slides. What do u mean by drying them extensively, the step to be done after AR or before AR.
I made AR on frozen sections on poly-Lysine coated slides. Some fall off, that happens. You need to dry them extensively (1h using a fan or O/N), that helps. Gelatin doesn't work as well, that's true. So cut sections, put on slide, dry, make AR.
Posted 24 January 2011 - 12:25 AM
The drying is after cutting and before AR.
Posted 26 January 2011 - 07:46 AM
When you cut the sections, are you floating them on warm water before mounting on the slides? This will really help get rid of any wrinkles.
We always leave the slides to dry AT LEAST overnight in a drying oven before doing anything else to them. I've never had any sections fall off when doing antigen retrieval after this.