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NO phosphorylatio shift


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#1 needhelp

needhelp

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Posted 20 January 2011 - 04:05 PM

Hi,

I have been trying to look at a phosphorylation shift using Drug treated Budding yeast cells,but with many attemps I have still not been able to sucessfully get my controls or samples to get working. I would appreciate any tips in general with looking at Phospho shifts. I;m using TCA preps & running it on 10% SDS gels after treating with Phsophotase etc.

Please any tips or advice is much appreciated.




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