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TCA protein extraction


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#1 molecule

molecule

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Posted 20 January 2011 - 03:57 PM

Hi ,
I have been trying to extract protein from Budding Yeast cells using TCA protein extraction, and concentrations of the protein is very low each time. I;m using around 25ml-50ml of culture to collect the cell samples. even more Volumes are not helping.  Are there any suggestions or protocols that are available specifically for a better protein extraction.




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