So, I'm annealing these 58bp oligos (18 of them in total from 36 ssDNA oligos), and just used TE pH 8 and it looks like it worked, since I cloned them into a vector and got many many more colonies in the insert plates vs the no-insert plate (about 10 fold more).
But it seems like everyone out there uses annealing buffers of some sort, which have a little bit of salt...
I haven't sequenced my colonies yet, so I cannot be 100% confident that it worked yet, but it definitely looks like it worked...
Are there any issues w/ just using TE? Isn't there enough residual salt after desalting in oligos ordered from, say IDT?
Annealing Oligos - Annealing Buffer vs TE vs Water
No replies to this topic