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Benzonase vs Micrococcal nuclease

Started by Sunnyside, Jan 20 2011 08:48 AM

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#1 Sunnyside

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Posted 20 January 2011 - 08:48 AM

I am doing protein purifications from mammalian cell lines looking for chromatin bound proteins. Our protocol uses benzonase nuclease to digest the DNA/RNA - other protocols use Micrococcal nuclease instead. Is there some advantage to one over the other? Does Benzonase digest chromatin?
Thanks

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