for me it is not clear, how to use the delta-delta Ct method, if my healthy controls not expressing this special gene (e.g. tumor marker)? I normally use a 45 cycle real time protocol, and some markers are undetermined.
hope tho get any suggestions, how to solve the problem,
thx
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2 replies to this topic
#2
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Enthusiast
Posted 20 January 2011 - 02:12 AM
You can insert maximal cycle number (45 in your case)as Ct value for non-expressing cells and proceed as usual.
#3
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Veteran
Posted 20 January 2011 - 03:33 AM
thx for recommendation.
another question arose: can I built a mean value of the delta Ct´s of all healthy controls to generate the delta delta ct for all samples (healthy & cancer patients)? = delta Ct of one sample minus the mean of the delta Cts of the healthy controls?
another question arose: can I built a mean value of the delta Ct´s of all healthy controls to generate the delta delta ct for all samples (healthy & cancer patients)? = delta Ct of one sample minus the mean of the delta Cts of the healthy controls?
