Posted 20 January 2011 - 01:03 AM
Posted 20 January 2011 - 06:59 AM
Posted 20 January 2011 - 09:14 AM
yup, green but only when it's bound to double-stranded DNA....red for SS DNA so we can also use it as a measure of DNA denaturation or fragmentation in cells...
Acridine orange is a metachromatic dye -- when it intercalates with DNA it fluoresces green (525nm), and when it interacts with RNA (by electrostatic attraction) it fluoresces red (>630nm).
Edited by casandra, 20 January 2011 - 09:24 AM.
- hobglobin, personal comment about my beauteous photo......