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acridine orange


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#1 tezuka

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Posted 20 January 2011 - 01:03 AM

When i fix the biofilms with glutaraldehyde and stain it with acridine orange, i expect it to turn red but in some biofilm it turns green. why is this so? what makes the color change?

#2 HomeBrew

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Posted 20 January 2011 - 06:59 AM

Acridine orange is a metachromatic dye -- when it intercalates with DNA it fluoresces green (525nm), and when it interacts with RNA (by electrostatic attraction) it fluoresces red (>630nm).

#3 casandra

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Posted 20 January 2011 - 09:14 AM

Acridine orange is a metachromatic dye -- when it intercalates with DNA it fluoresces green (525nm), and when it interacts with RNA (by electrostatic attraction) it fluoresces red (>630nm).

yup, green but only when it's bound to double-stranded DNA....red for SS DNA so we can also use it as a measure of DNA denaturation or fragmentation in cells...

Edited by casandra, 20 January 2011 - 09:24 AM.

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