When i fix the biofilms with glutaraldehyde and stain it with acridine orange, i expect it to turn red but in some biofilm it turns green. why is this so? what makes the color change?
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acridine orange
Started by tezuka, Jan 20 2011 01:03 AM
2 replies to this topic
#2
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Posted 20 January 2011 - 06:59 AM
Acridine orange is a metachromatic dye -- when it intercalates with DNA it fluoresces green (525nm), and when it interacts with RNA (by electrostatic attraction) it fluoresces red (>630nm).
#3
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carpe diem by the jugulum
Posted 20 January 2011 - 09:14 AM
HomeBrew, on 20 January 2011 - 06:59 AM, said:
Acridine orange is a metachromatic dye -- when it intercalates with DNA it fluoresces green (525nm), and when it interacts with RNA (by electrostatic attraction) it fluoresces red (>630nm).
yup, green but only when it's bound to double-stranded DNA....red for SS DNA so we can also use it as a measure of DNA denaturation or fragmentation in cells...
Edited by casandra, 20 January 2011 - 09:24 AM.
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