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Removing adherent cells from Matrigel


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#1 kieselgur

kieselgur

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Posted 19 January 2011 - 09:51 PM

Hallo,


I'm new to tissue culture and matrigel. I am currently working with adherent cells and I am interested in quantifying the residual amount of matrigel left in the plate after incubation. I am concerned about using trypsin because it may degrade matrigel?

I have tried incubation with EDTA for up to 1 hour at 37 C and it doesn't work either. Is there another way I could use to overcome this problem. Or some basic technique that is so common knowledge that people don't bother publishing about it?

I'm lost :(




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