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protein overexpression in HeLa cells


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#1 mnohreally

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Posted 19 January 2011 - 12:37 PM

Hello.
I'm trying to overexpress a protein (wip1) in HeLa cells, using cDNA.
cDNA I'm getting comes in pCMV-sport6 vector, which is a mammalian expression vector.

I'm wondering if direct transient transfection of HeLa cells with the pCMV-sport6 with my cDNA would do the overexpression.

Can anyone help?

#2 bob1

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Posted 19 January 2011 - 02:54 PM

It should work, the CMV is a viral promoter designed for over-expression of proteins running off the promoter.




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