TBE Acrylamide/Formamide gels running slowly
Posted 19 January 2011 - 10:40 AM
We have swapped out Acrylamide and Urea brands, and tried fresh APS, TEMED and TBE, with no luck.
We run large scale PAGE gels (BioRad SequiGen 38x50cm) for DNA SSR analysis.
Usually 1.75h @ 110watts (1800V-2800V)
7% (19:1 acrylamide:Bis)
We use both "regular" acrylamide gels, as well as acrylamide+formamide, with the same results.
Posted 19 January 2011 - 11:30 AM
maybe the limits are changed or not outputting what you expect or the electrodes damaged or dirty.
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 19 January 2011 - 11:41 AM
Unfortunately, it happens with all 3 packs, as well as the new and old base units.
Posted 19 January 2011 - 02:19 PM
Posted 19 January 2011 - 02:42 PM
Our cartridges are due for a change (thanks for the reminder)... but I can't imagine they're that bad yet.