I have recently been told I cannot refer to KD values calculated through binding assays withour calling them apparent KDs (or KDapp). I think this is because a standard binding assay only measures association rates and not disassociation rates. Can anyone confirm this? I've been googling for half an hour now and I can't find any good explanations for the use of the term apparent!
Edited by Penguin, 19 January 2011 - 05:03 AM.