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HRM genotyping - two neighboring SNPs


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#1 cells

cells

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Posted 17 January 2011 - 12:45 PM

Hi,

I'm etremely new to genotyping and HRM so I need some help if someone has experience in this area.

I have a bunch of DNA from different patient samples and my PI has asked me to find the frequency of specifc SNPs of a gene. We only have the regents to perform HRM (no TaqMan at the moment) so I'm wondering how the primer design and analysis in HRM will work. I'm specifically interested in knowing how you would go about designing primers to genotype a specifc SNP if there is another SNP present right beside it.

So, I have:

-------*&---------
* is the SNP of interest
& is another SNP right beside the SNP of interest


Is there a specifc way to use the HRM method here to genotype the SNP of interest?

Anyways, if anyone can help me out here or provide any sources then that would be really appreciated.

Thanks!




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