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Random mutagenic PCR using Vent


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#1 Mattise

Mattise

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Posted 17 January 2011 - 12:09 PM

I was wondering if anyone has a protocol for random PCR mutagenesis using Vent. I was trying normal taq, which is what is often used for random mutagenesis, but I can't get taq to work under any circumstance, even as a positive control, so I wanted to move to vent.




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