Bacteria to Cell Culture
Posted 16 January 2011 - 05:58 AM
I've started to a new research about cytokine expression. I'll add bacteria to osteoblast cell culture to see cytokine expression. But I dont know How many bacteria I should add to how many cell? I read articles they say 50, 100 MOI . What is this MOI? How can I calculate it?
Please help me I have to do the experiment tomorrow.
Posted 16 January 2011 - 05:21 PM
Posted 16 January 2011 - 06:31 PM
So in your case, number of bacteria per osteoblast cell.
Eg. for an MOI of 50 you would have 50 bacteria per single osteoblast.