Hi,
I've started to a new research about cytokine expression. I'll add bacteria to osteoblast cell culture to see cytokine expression. But I dont know How many bacteria I should add to how many cell? I read articles they say 50, 100 MOI . What is this MOI? How can I calculate it?
Please help me I have to do the experiment tomorrow.
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#2
bob1
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Posted 16 January 2011 - 05:21 PM
MOI= multiplicity of infection. I'm not a microbiologist, so I don't know how to calculate it, other that it will be a measure of the number of cells needed to cause infection in a particular system or volume.
#3
leelee
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Posted 16 January 2011 - 06:31 PM
As bob1 said, MOI is multiplicity of infection. It means the number of infectious units per host/target unit.
So in your case, number of bacteria per osteoblast cell.
Eg. for an MOI of 50 you would have 50 bacteria per single osteoblast.
So in your case, number of bacteria per osteoblast cell.
Eg. for an MOI of 50 you would have 50 bacteria per single osteoblast.
