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Bacteria to Cell Culture


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#1 kubic

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Posted 16 January 2011 - 05:58 AM

Hi,

I've started to a new research about cytokine expression. I'll add bacteria to osteoblast cell culture to see cytokine expression. But I dont know How many bacteria I should add to how many cell? I read articles they say 50, 100 MOI . What is this MOI? How can I calculate it?

Please help me I have to do the experiment tomorrow.

#2 bob1

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Posted 16 January 2011 - 05:21 PM

MOI= multiplicity of infection. I'm not a microbiologist, so I don't know how to calculate it, other that it will be a measure of the number of cells needed to cause infection in a particular system or volume.

#3 leelee

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Posted 16 January 2011 - 06:31 PM

As bob1 said, MOI is multiplicity of infection. It means the number of infectious units per host/target unit.
So in your case, number of bacteria per osteoblast cell.

Eg. for an MOI of 50 you would have 50 bacteria per single osteoblast.




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