3 replies to this topic

[DNA]

Hello Everyone,

I am working with a large number of Human GABAA receptors subunits.I have made glycerol stocks of all of them. However I want to preserve them on filter papers and actually make a folder of them.That will make shipment etc easy too.

Has anyone attenmpted this? Any information, what sort of filter paper should be used etc.
A detail description of protocol/method will be appreciated.

Thanks

[bob1]

Suspend DNA in tris-Cl pH 7-7.5, label filter paper (usually put a small circle in pencil, within which you should add the DNA), add a couple of ug to filter paper (whatman's no.1 works well), dry out, store.

[DNA]

Thanks Bob.. Was busy in my thesis so its been a long time since I posted on bioforum.
I will make that folder of plasmid DNA's now.

[leelee]

We use Whatman FTA nucleic acid storage paper for DNA......