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How to store plasmid DNA on filter paper


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#1 DNA

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Posted 15 January 2011 - 06:10 PM

Hello Everyone,

I am working with a large number of Human GABAA receptors subunits.I have made glycerol stocks of all of them. However I want to preserve them on filter papers and actually make a folder of them.That will make shipment etc easy too.

Has anyone attenmpted this? Any information, what sort of filter paper should be used etc.
A detail description of protocol/method will be appreciated.

Thanks

#2 bob1

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Posted 15 January 2011 - 06:26 PM

Suspend DNA in tris-Cl pH 7-7.5, label filter paper (usually put a small circle in pencil, within which you should add the DNA), add a couple of ug to filter paper (whatman's no.1 works well), dry out, store.

#3 DNA

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Posted 16 December 2011 - 07:52 PM

Thanks Bob.. Was busy in my thesis so its been a long time since I posted on bioforum.
I will make that folder of plasmid DNA's now.

#4 leelee

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Posted 16 December 2011 - 10:05 PM

We use Whatman FTA nucleic acid storage paper for DNA......




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