DNA extraction from plated colonies
Posted 14 January 2011 - 11:16 AM
I'm looking for a protocol to directly extract DNA from E.Coli (DH5Alpha) colonies on Agar+ Amp plates without growing them overnight in LB broth. The reason for this is that while the bacteria grows well on the plates it does not do so in the broth, so we're trying to side step this problem. Any suggestions?
Posted 14 January 2011 - 04:27 PM
Posted 14 January 2011 - 11:52 PM
Edited by pDNA, 14 January 2011 - 11:53 PM.
Posted 15 January 2011 - 01:49 AM
If you still certain that you want to extract DNA from the colonies - we've used Templifee kit from Amersham couple of years ago.
It allows you to prepare sufficient amount of DNA for sequencing.
Hope it works for you.