Routinly, I perform IF staining by incubate the cryosections with diluted primary antibody at RT overnight. Now I want to reuse my antibody, so I want to know how long the diluted primary antibody have it's binding capacity. I dilute 1st Ab with PBS supplimented with 0.05% tween-20.
Thank you for your help!
Edited by Flint, 14 January 2011 - 08:07 AM.