topic and cell culture
Posted 14 January 2011 - 01:40 AM
after 1 day they are showing lysis.
is it normal ?
or is it happening due to some other infection
Posted 15 January 2011 - 06:39 PM
Posted 18 January 2011 - 03:51 AM
started this for first time in laboratory.
therefore, no experienced person available.
cells are cultered in EMEM media, 27 deg , incubation with virus variable.
chik virus does not cause any cytopathic effect in mosquito host.
so, it should multiply peacefully in mosquito cell line without causing lysis