High mRNA but no protein expression
Posted 13 January 2011 - 09:45 AM
i have performed RT PCR and i have found for my gene that the mRNA levels are very high.the problem is that the protein is not expressed in the cells!!!any ideas why?the sequencing is ok,the western blots do not detect protein but the RT PCr gave a high amount of mRNA.thank you
Posted 13 January 2011 - 02:41 PM
Posted 14 January 2011 - 12:47 AM
i have two probes:one whole receptor and the receptor with a depleted sequence!in western blots i can detect the whole receptor but not the depletion!in LSM microscopy i can see the receptor(tagged with GFP) on the membrane but the depleted Receptor is all over the cytosol the GFP stain!depletion seems so to be fast degraded but RT PCR gives me high amounts of this mRNA even higher from the normal receptor.any ideas?
Posted 14 January 2011 - 04:54 AM
Posted 14 January 2011 - 05:42 AM
Posted 15 January 2011 - 06:53 PM
Posted 16 January 2011 - 12:32 AM
Posted 17 January 2011 - 02:22 AM
Is the GFP fused to the N- or C-terminus of your receptor?
Posted 17 January 2011 - 02:50 AM
Posted 17 January 2011 - 04:14 AM
Posted 17 January 2011 - 04:49 AM