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Protein assay


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#1 My

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Posted 29 May 2002 - 04:05 AM

I'm doing a subcellular fractionation using a percoll gradient. I don't know which protein assay to use. I have read that Bradford doesn't work when you have percoll in your samples. Anyone who knows??

#2 scogne

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Posted 30 May 2002 - 10:24 PM

Hello,

try BCA assay from Pierce (www.piercenet.com), it is not time consuming and only a few chemicals does interfere at so high concentration.





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