
Transfer buffer as running buffer?
#1
Posted 12 January 2011 - 12:31 PM
#2
Posted 12 January 2011 - 12:37 PM
#3
Posted 12 January 2011 - 12:46 PM
When you, finish tell me about how run the gel.
good luck!
#4
Posted 12 January 2011 - 12:48 PM
#5
Posted 12 January 2011 - 02:49 PM
#6
Posted 12 January 2011 - 05:54 PM
It is best to run the gel again, but it may run OK, though the proteins might not migrate where you expect them to.
#7
Posted 09 November 2012 - 06:46 AM
Any suggestions or comments?
#8
Posted 27 November 2012 - 02:52 AM
I did the same mistake today and I've run the gel with Transfer buffer instead of Running buffer. We don't use any SDS in the transfer buffer. But the marker bands and the Sample buffer was run perfectly as usual without any problem(by observation of the gel). And I am transferring now with transfer buffer to PVDF. I don't know how the blot is gonna look like.
Any suggestions or comments?
luckily the gel was run fine and even the western blot turned out to be good. So I guess using transfer buffer for running is not such a big mistake.