Transgenic mouse model to measure Ca2+ in confocal
Posted 13 January 2011 - 07:53 AM
hmm, not sure if this will help...
..I don't think Ive seen much on FRETing neural tissues, you might want to check out other models such as alzheimers, maybe it will lead to a clever solution..
Edited by Zymech, 13 January 2011 - 07:54 AM.
Posted 13 January 2011 - 10:24 AM
then heaven will be yours, before you meet your end
Posted 19 February 2011 - 07:14 AM
I'm not completely sure why you're looking at transgenic models for intracellular calcium measurements. Fura-2 is a standard ionophore for measuring calcium levels in cells in response to chemical stimuli. You might also look at Cameleon, a CamKII-GFP hybrid protein that can be transfected or transduced into cells to visualize calcium concentration.