3 replies to this topic

[Inmost sun]

does anyone know a transgenic mouse model to measure Ca2+ levels in neuronal tissue by confocal microscopy (probably by FRET? any hint will help...

[Zymech]

I suppose this is a culture then, and not a mouse on the table.

o.O

hmm, not sure if this will help...

http://www.springerl...&page=1&locus=5

..I don't think Ive seen much on FRETing neural tissues, you might want to check out other models such as alzheimers, maybe it will lead to a clever solution..

Edited by Zymech, 13 January 2011 - 07:54 AM.

[gfischer]

I'm not completely sure why you're looking at transgenic models for intracellular calcium measurements.  Fura-2 is a standard ionophore for measuring calcium levels in cells in response to chemical stimuli.  You might also look at Cameleon, a CamKII-GFP hybrid protein that can be transfected or transduced into cells to visualize calcium concentration.

[Inmost sun]

that is the thing: If you have mutant mice stably expressing those calcium-senstive fluorescent proteins you can measure calcium in living mice...

gfischer, on 13 January 2011 - 10:24 AM, said:

I'm not completely sure why you're looking at transgenic models for intracellular calcium measurements.  Fura-2 is a standard ionophore for measuring calcium levels in cells in response to chemical stimuli.  You might also look at Cameleon, a CamKII-GFP hybrid protein that can be transfected or transduced into cells to visualize calcium concentration.