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[anonymous]

Hello, I am after a quick and simple method for isolating human chondrocytes, synovial fibroblasts and macrophages from joint tissue. Thanks!!

[Anne]

Culturing human chondrocytes; primary culture. First you need to get a good sample of chondrocytes, perhaps by arthroscopy. Do take care of aseptics. Then you have to lysate the sample in order to get the cells. Use medium with glucose,serum ( from the doner ), antibiotics. Non-essential amino acids are not recuired, if you don't culture cells for long.
Follow all other usual methods of cell culture. Good luck!
Details can be found in articles about topic. ( PubMed is a good source )