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housekeeping genes in PCR


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#1 vitalgene

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Posted 12 January 2011 - 05:27 AM

Please name the housekeeping genes one can use for (mammalian) PCR analysis ?

#2 Curtis

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Posted 12 January 2011 - 06:30 AM

Actin is the most famous one, buy the primers from Sigma,

Can also try GAPDH, but depends on the experiment too. For my case Actin didn't work fine so I ordered other housekeeping gene, one of them was 18S if I'm not wrong




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