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Experience with Yeast Contamination?

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#1 Timothy007



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Posted 11 January 2011 - 08:23 PM

Does anyone have experience with yeast contamination?

I suspect my HEK293TF culture get contaminated by yeast. They existed as round, ovoid, solid, small, yellowish distinguishable single colonies.

No change of pH and turbidity was observed. But the growth rate of cell culture reduced significantly. It usually takes me 1 day to attain 80% confluence in 35mm plate seeding at 1x10^5 cell density. Now, it only gives 30% confluence.

Having said that, the colony are very similar in size with some small cryo-precipitates of serum.

I am planing to use Amphotericin B to "cure" the problem. But I am wondering if this treatment is possible to "cure" it....

My questions are:

1. Does anyone have experience with yeast contamination?
2. What protocol you used?
3. How many passage required ?
4. Is that possible to "cure" the problem?

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