Posted 11 January 2011 - 08:02 AM
The lab that I work in has had contamination problems many times in the past yet it appears (at least to me) to come and go cyclically. We switched from Pen/Strap to Gentamicin for our antibiotic in our media awhile back, we've had the cell culture room cleaned, we spray with an ethanol and benzalkonium chloride mixture, etc. Still, no matter what we do, we still get contamination, especially after transfections (we generally use Bosc23 cells). With our strongest magnification on our scope, we can see small, black, moving squares, so it seems to be bacterial as opposed to fungal, but at this point, we really don't know. This contamination issue has pushed me (and others) back a long time. Some think that it could be the instruments (i.e. pipettors, etc.), but we can't autoclave them (someone tried and they didn't work afterward). We've tried to clean things with bleach every now and then, but that still doesn't seem to do the trick.
Does anyone have advice?