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DNA isolation


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#1 pavi

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Posted 10 January 2011 - 06:46 PM

Does the concentration of alcohol added affect the amount of DNA that precipitates out?
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#2 Ameya P

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Posted 10 January 2011 - 09:30 PM

Does the concentration of alcohol added affect the amount of DNA that precipitates out?


It depends on what concentration is your alcohol at? Say, if you added 60% or 80% alcohol instead of 70% alcohol, it would not make a huge difference, but if you added like 20% alcohol, then the DNA that would precipitate out would be very little because it would dissolve in the water.

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#3 ElHo

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Posted 12 January 2011 - 03:23 AM

The most important thing for ethanol precipitation is to end up with a final ethanol concentration of 70%. This is usually achieved by adding 2.5 volumes of 100% ethanol to the DNA solution you want to precipitate.
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