min6 cells immunofourescence staining
Posted 10 January 2011 - 08:40 AM
I have just begun to learn min6 cells staining but i couldn'i take any results, i can not see green flourescence, primary ab-secondary ab is compatible nad has no problem, give me please advise?
Posted 10 January 2011 - 02:43 PM
Posted 20 January 2011 - 04:23 PM
When my slides are ready I put a drop of DAPI on the slide and then put it onto a glass slide. You can use clear nail polish to seal the edges of your slides. If you store your slides at -20C. They should be alright for a while.
Posted 24 January 2011 - 11:34 AM
Posted 24 January 2011 - 02:26 PM
So so Thank you for your reply, i will try as you said, because my reagents work perfect and i just follow the protocol..i hope it will work at this time..
btw, make sure u work in very dim light bc the fluorescent proteins will be bleached out by light. i usually worked ina room w/ the light off and cracked the door enough so I could see what i was doing.