high background after ECL exposition
Posted 10 January 2011 - 08:32 AM
i tried to scan again the filter on the storm, without using ECL Plus again, so, i could see a little bit better signal.
saturation still present. i'm trying to block again the filter and scan on the storm without any other incubation with antibody or ECl exposition!
do you think is it correct to do it??
Posted 11 January 2011 - 02:10 PM
Posted 12 January 2011 - 03:41 AM
From this point, I do again the secondary antibody incubation, washings and ECL. The fresh secondary, still recognizes some primary but the background now is less (as saturated by previous silent antibodies).
Sometimes works, sometimes not.
Posted 12 January 2011 - 02:52 PM