Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Circular Dichroism and disordered proteins

  • Please log in to reply
1 reply to this topic

#1 Penguin



  • Active Members
  • PipPipPipPipPip
  • 64 posts

Posted 10 January 2011 - 08:23 AM


I have never done any CD and only have a vague idea of how it works, but in my PhD thesis (which is due in at the end of the month!!!!) I mention that the protein I work with is intrinsically disordered, some of the evidence being a CD minimum at 200 nm. I've tried searching for explanations for why a minimum at 200 nm means disorder but find that most guides to CD are inpenetrable for non-experts!! I assume that unfolded polypeptides don't absorb well at 200 nm but why??

If I am asked about this in my viva I'm not sure what to say - any ideas??


#2 Prep!


    Am I me???!!!

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 517 posts

Posted 24 January 2011 - 07:55 PM

i have never done CD... but of wat i have read many articles and tutorials suggeest that getting a value at 200 nm is anyways difficult because of the buffers used in the assay...
Support bacteria - They are the only culture some people have!!!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.