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[Penguin]

Hi,

I have never done any CD and only have a vague idea of how it works, but in my PhD thesis (which is due in at the end of the month!!!!) I mention that the protein I work with is intrinsically disordered, some of the evidence being a CD minimum at 200 nm.  I've tried searching for explanations for why a minimum at 200 nm means disorder but find that most guides to CD are inpenetrable for non-experts!!  I assume that unfolded polypeptides don't absorb well at 200 nm but why??

If I am asked about this in my viva I'm not sure what to say - any ideas??

Thanks,
P

[Prep!]

i have never done CD... but of wat i have read many articles and tutorials suggeest that getting a value at 200 nm is anyways difficult because of the buffers used in the assay...