Posted 10 January 2011 - 07:27 AM
I have two question about gel electrophoresis, the first one is:
if I try to keep the gel after running to save my sample for long time, how can i keep it?
And the second question is:
If I try to cut specific DNA band from the gel, then at which voltage I should run the gel? to make long distance between bands..
Posted 10 January 2011 - 10:39 PM
Posted 10 January 2011 - 11:14 PM
But if you must keep it in the gel, then I would suggest at least excising the band and then freezing until you are ready to proceed.
Keep in mind your gel will not be nuclease free....
Posted 13 January 2011 - 03:58 AM
Voltage and time for the gel depends on what size bands you are cutting out, and the size of any other bands that will also be on the gel. the smaller the bands you are looking for the shorter time you can run for, heavy bands take much longer to run to distinguish between sizes. If your band of interest is 3.5kb but there is an irrelevant band at 3kb, then you may need to run for linger to separate them more than if the only contaminants were of a much heavier size.
Posted 16 July 2011 - 06:58 PM