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plasmid DNA versus genomic DNA

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#1 claritylight



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Posted 09 January 2011 - 09:38 PM

why is plasmid DNA more concentrated than genomic DNA? I was told that in order to PCR plasmid DNA, it has to be at a lower number of cycles than what is done for genomic DNA but I don't understand how plasmid DNA is much more concentrated than genomic DNA in the first place. i appreciate any help.

#2 mdfenko


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Posted 10 January 2011 - 08:48 AM

they're talking about molar concentration. each cell has one copy of genomic dna but can contain many copies of plasmid dna.
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