What cause "downshift" in SDS-PAGE?
Posted 09 January 2011 - 07:44 PM
Everybody knows the most obvious one is phosphatase (or dephosphrylation). I just wanna if there's any possbile mechanism to cause this observation.
Posted 10 January 2011 - 01:35 AM
Posted 10 January 2011 - 06:13 AM
Actually itīs phosphorylation, not dephosphorylation that causes proteins to migrate faster.
not to my understanding. Phosphorylation should cause a upshifted band.
Posted 10 January 2011 - 10:44 AM
phosphorylation will add very little to molecular weight but will increase negative charge on molecule (even in the presence of sds). you can enhance the downshift caused by phosphorylation by running the protein on urea-page.
Edited by mdfenko, 10 January 2011 - 10:47 AM.
talent does what it can
genius does what it must
i used to do what i got paid to do
Posted 10 January 2011 - 12:42 PM
Posted 24 January 2011 - 07:49 PM