Trying to amplify the 3' end of Prolyl endopeptidse gene from cDNA.. need su
Posted 09 January 2011 - 07:10 PM
Posted 09 January 2011 - 10:53 PM
Posted 10 January 2011 - 11:16 AM
I made the cDNA using the Clonetech SMARTer RACE cDNA synthesis kit for 5' and 3' end amplification. But.. that worked only for 5' end.
For the expression of your desired gene full length gene isolation which is very specific with 3' and 5' SMART RACE kit provided by various firms is useful.