i would like to know that will the linearized plasmid still can grow after introduction into competent cells?
Edited by chi, 09 January 2011 - 05:37 PM.
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#1
chi
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Posted 09 January 2011 - 04:28 PM
Hi, everyone,
i would like to know that will the linearized plasmid still can grow after introduction into competent cells?
i would like to know that will the linearized plasmid still can grow after introduction into competent cells?
#2
pDNA
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Posted 10 January 2011 - 01:33 PM
No, since linear plasmid DNA can not be replicated.
Regards,
p
Regards,
p
#3
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Posted 10 January 2011 - 06:14 PM
pDNA, on 10 January 2011 - 01:33 PM, said:
No, since linear plasmid DNA can not be replicated.
Regards,
p
Regards,
p
is that mean the cell can take up the linearized plasmid but the plasmid cannot replicate inside cell?
and also, why the plasmid cannot replicate?
#4
pDNA
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Posted 10 January 2011 - 10:27 PM
linear DNA can be taken up by cells, that is not the problem ...the problem is that replication is dependent on a circular molecule, and a double strand break can not ligated by E. coli.
Regards,
p
Regards,
p
