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why the cells transformed with linearized plasmid cannot grow?


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#1 chi

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Posted 09 January 2011 - 04:28 PM

Hi, everyone,

i would like to know that will the linearized plasmid still can grow after introduction into competent cells?

Edited by chi, 09 January 2011 - 05:37 PM.


#2 pDNA

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Posted 10 January 2011 - 01:33 PM

No, since linear plasmid DNA can not be replicated.

Regards,
p

#3 chi

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Posted 10 January 2011 - 06:14 PM

No, since linear plasmid DNA can not be replicated.

Regards,
p

thx.

is that mean the cell can take up the linearized plasmid but the plasmid cannot replicate inside cell?
and also, why the plasmid cannot replicate?

#4 pDNA

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Posted 10 January 2011 - 10:27 PM

linear DNA can be taken up by cells, that is not the problem ...the problem is that replication is dependent on a circular molecule, and a double strand break can not ligated by E. coli.

Regards,
p




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