Catechol 1,2 Dioxygenase Activity in Bacterial Isolate
Posted 09 January 2011 - 11:24 AM
Posted 09 January 2011 - 11:30 AM
Posted 09 January 2011 - 11:34 AM
How do you know the bacterium is indeed growing on the benzoic acid as the sole carbon source?
Bacterium has been grown in a mineral salts medium containing benzoic acid as the sole source of carbon and energy. Growth has been observed by measuring the increase in optical density at 600 nm, increase in cell numbers and by protein determination.
Posted 15 January 2011 - 10:25 AM
(you need to be 100% sure that they grow indeed on the benzoic acid and not a c source they have from another petri dish or medium you grow them in before putting them on a benzoic acid medium).
This can be due the fact they do not grown well on benzoic acid as the sole carbon source.
They might need to adjust.
+ why dont you test them growing on a good (rich medium) with benzoic acid as the c source but for the rest a good medium (I am thinking you use a very minimal medium?)
And is it aereated ? You are are keeping the pH constant?