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Transformation and selection of yeast with pRS426


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#1 autophagator

autophagator

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Posted 08 January 2011 - 11:48 AM

Hi all°
I am performing my doctorate with yeast S.cerevisiae. Its a completely new organism for me, and now I am having troubles with him/her/she/he/it whatever.. Recently, I did transform a simple mutant (ura+) to create a double mutant. The simple mutant was ura+ ( with pRS426 plasmid) and grows normally in SC-ura plaques. However, after transformation (with other DNA and selection non relationated), the double mutant donít grow in liquid media SC-ura. Why?? I donít understand°° I did repeat the test several time, and still happens. Can anybody suggest me an idea, test, paper, link o something to know what happens here????
Thank a lot in advance.




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