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Can I use frozen culture supernatant for ELISA

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#1 TracyDuke



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Posted 07 January 2011 - 10:00 AM

I am trying to measure IgG secretion of cells in culture. I need to take samples every 2 days during an 8-day period. I am wondering if I could collect the samples and freez them untill the end of the culture and then measure them all at once? Thank you!

#2 protolder



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Posted 10 January 2011 - 11:03 PM

Hola, Yes you can, at the time to do an ELISA, you collect all the store samples, and commercial controls to follow a purification or a cellular production. The problem would be that the samples would be degradated, but freezing isnīt the case excep in some repeated freezing and tawing process. Buena suerte

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