Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account

- - - - -

NS-1 myeloma line


  • Please log in to reply
2 replies to this topic

#1 Chelo

Chelo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 92 posts
3
Neutral

Posted 07 January 2011 - 04:44 AM

Dear all,

I have just started to work with NS-1 myeloma cells in my lab (so far, we were working with SP2O). I am culturing the cells and I found that when doing so in 48-well plates the cells adhere to the plastic and change their shape. In concrete, many of them stop being round to get a stick shape. I never saw this with the SP2O cells so I wanted to ask if anyone working with NS1 could tell me if this phenomenon is normal.

#2 BioMiha

BioMiha

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 225 posts
8
Neutral

Posted 07 January 2011 - 06:52 AM

We have been working with NS-1 for a very long time and we have never seen anything like that. Are you sure you have the NS-1?

#3 Chelo

Chelo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 92 posts
3
Neutral

Posted 07 January 2011 - 11:47 AM

View PostBioMiha, on 07 January 2011 - 06:52 AM, said:

We have been working with NS-1 for a very long time and we have never seen anything like that. Are you sure you have the NS-1?
Yes, we purchased it from Sigma. I will check for mycoplasma contamination. Thx!




Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.