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NS-1 myeloma line


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2 replies to this topic

#1 Chelo

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Posted 07 January 2011 - 04:44 AM

Dear all,

I have just started to work with NS-1 myeloma cells in my lab (so far, we were working with SP2O). I am culturing the cells and I found that when doing so in 48-well plates the cells adhere to the plastic and change their shape. In concrete, many of them stop being round to get a stick shape. I never saw this with the SP2O cells so I wanted to ask if anyone working with NS1 could tell me if this phenomenon is normal.

#2 BioMiha

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Posted 07 January 2011 - 06:52 AM

We have been working with NS-1 for a very long time and we have never seen anything like that. Are you sure you have the NS-1?

#3 Chelo

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Posted 07 January 2011 - 11:47 AM

We have been working with NS-1 for a very long time and we have never seen anything like that. Are you sure you have the NS-1?

Yes, we purchased it from Sigma. I will check for mycoplasma contamination. Thx!




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