NS-1 myeloma line
Posted 07 January 2011 - 04:44 AM
I have just started to work with NS-1 myeloma cells in my lab (so far, we were working with SP2O). I am culturing the cells and I found that when doing so in 48-well plates the cells adhere to the plastic and change their shape. In concrete, many of them stop being round to get a stick shape. I never saw this with the SP2O cells so I wanted to ask if anyone working with NS1 could tell me if this phenomenon is normal.
Posted 07 January 2011 - 06:52 AM
Posted 07 January 2011 - 11:47 AM
Yes, we purchased it from Sigma. I will check for mycoplasma contamination. Thx!
We have been working with NS-1 for a very long time and we have never seen anything like that. Are you sure you have the NS-1?